Views: 0 Author: Site Editor Publish Time: 2022-12-19 Origin: Site
When doing nucleic acid testing, I believe that many medical sampling personnel, registration personnel, and transportation personnel have been curious about this question. What is the red liquid of cryogenic vials? Will it be harmful to the body if it is accidentally touched? How to deal with it?
The red liquid in cryovial tubes for nucleic acid detection is a virus preservation solution, which is suitable for the collection, storage, and transportation of various common virus samples such as new coronavirus, influenza virus, and hand-foot-mouth virus, and is convenient for subsequent nucleic acid extraction or purification experiments.
The main components of the inactivated virus preservation solution are guanidine salt, bis-imidazolidine, phenol red, buffer, etc.
Guanidine salt is one of the important components of virus preservation solution. It can destroy the virus surface protein coat, making the virus inactive and infective. Commonly used guanidine salts are guanidine isothiocyanate or guanidine hydrochloride. Relevant studies have shown that inactivated preservation tubes containing guanidinium salts can effectively preserve a variety of viral nucleic acids. For the 20-in-1 mixed collection collection tube, it is required that the tube contains 11-12 ml of guanidine salt or other effective virus inactivator preservation solution. Guanidine salt will eventually be hydrolyzed into ammonia and urea in aqueous solution, most of which will be excreted with urine, which is generally harmless to the human body.
The preservation solution also contains a certain amount of bis-imidazolidine, which is usually used as a preservative because of its bactericidal and antibacterial effects. It is also added to some of our commonly used facial masks and lotions. As long as the dosage is within the allowable range, it will not Harm to human body.
Phenol red is a kind of acid-base indicator, it is red in neutral environment, which is the "red liquid" we usually see! Phenol red turns purple when it meets alkali, and turns yellow when it meets acid. If the preservation solution is contaminated by bacteria (commonly due to the failure of the preservation solution or bacteria in the sample), the pH value of the liquid will drop, and it will turn from red to yellow.
In fact, the original nucleic acid preservation solution was colorless, and now the red nucleic acid preservation solution is intentional. The main reason is that the deep-well plates used to store samples in the laboratory are colorless, and nucleic acid testing personnel need to wear protective face shields or goggles when testing, and it is very easy to add wrong wells through a layer of glass bit or leak plus hole bit. Therefore, in the "nucleic acid cryogenic tubes" developed later, a phenol red indicator was deliberately added, which not only facilitates the operation of the staff, but also makes it easier to distinguish different detection types.
Speaking of this, we can basically understand that the main function of the "red liquid" is to inactivate viruses, sterilize and inhibit bacteria, maintain pH and ensure the stability of nucleic acids in viruses, and generally do not cause harm to the human body. Even if you accidentally get it on your hands, don't worry too much, just rinse it off with clean water.
For more knowledge, please contact cryogenic vials supplier - MGG group.
